GPI-Anchored proteins (GPI-APs) can be exogenously transferred onto bilayer membranes both in vivo and in vitro. while the mechanism by which this transfer process occurs is unknown. In this work. we used atomistic molecular dynamics simulations and free energy calculations to characterize the essential influence of cholesterol on insertion of the GPI anchors into plasma membranes. https://www.pinkboutiques.shop/product-category/denim-jumpsuits/